Journal: PLoS ONE

Article Title: The fucose salvage pathway inhibits invadopodia formation and extracellular matrix degradation in melanoma cells

doi: 10.1371/journal.pone.0199128

Figure Lengend Snippet: A, schematic illustration showing the modification of glycans by α-1,2, α-1,3 and α-1,4 branched fucosylations. B, the effects of L-fucose and fucosidase treatment on cell surface α1–2 fucosylation, as determined by UEA-1 staining and flow cytometry. C, quantification of the effects of α-1,2 fucosidase treatment on fucose-mediated inhibition of invadopodium formation in WM793 cells. D, representative invadopodium assay images showing the effect of α-1,2 fucosidase treatment on FUK-mediated inhibition of invadopodium formation in WM793 cells. E and F, quantitation of the effect of α-1,2 fucosidase treatment on the invadopodium number per cell ( E ) and the proportion of invadopodia positive cells ( F ) in control or FUK OE WM793. G and H, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( G ) and proportion of invadopodia positive cells ( H ) in control or fucose-treated WM793. I and J, quantitation of the effect of α-1,3/4 fucosidase treatment on the average invadopodium number per cell ( I ) and proportion of invadopodia positive cells ( J ) in control or FUK-overexpressed WM793. ** and *** indicates p<0 . 01 and 0 . 001 , respectively, as determined by two-tailed, two sample t-test ( C, E ) or two-tailed Fisher’s exact test ( F, G ). ns, not statistically significant. Numbers in parentheses indicates the number of cells used in quantitation. Representative results from at least three independent replicates were presented.

Article Snippet: Cells were washed twice in 1xPBS and stained with 0.2μg/mL FITC-UEA-1 (Vector Labs, FL1061) for 1 hour at room temperature and protected from light.

Techniques: Modification, Staining, Flow Cytometry, Inhibition, Quantitation Assay, Two Tailed Test